3TZ9

Kinase domain of cSrc in complex with RL130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	CCD
Collection date	2010-05-23
Detector	MARRESEARCH
Wavelength(s)	1.003900
Spacegroup name	P 1
Unit cell lengths	42.020, 63.420, 74.200
Unit cell angles	79.60, 87.89, 89.98

Refinement procedure

Resolution	41.990 - 3.100
R-factor	0.2162
Rwork	0.211
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f3v
RMSD bond length	0.012
RMSD bond angle	1.363
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	3.200
High resolution limit [Å]	3.100	16.000	3.100
Rmerge	0.062	0.032	0.205
Rmeas		0.045	0.290
Number of reflections	12539	94	1166
<I/σ(I)>	9.61	23.4	4
Completeness [%]	91.8	93.1	93.7
Redundancy	1.79		1.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	10% PEG 20000, 15% glycerol, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K