3TZ1

Crystal structure of the Ca2+-saturated C-terminal domain of Akazara scallop troponin C in complex with a troponin I fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2006-03-03
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	32.130, 42.151, 59.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.321 - 1.800
R-factor	0.2047
Rwork	0.203
R-free	0.23670
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.053
Number of reflections	8036
<I/σ(I)>		5.5
Completeness [%]	99.5	97.8
Redundancy	3.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	30% PEG 4000, 0.1 M sodium acetate pH 4.6 and 0.2 M ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	30% PEG 4000, 0.1 M sodium acetate pH 4.6 and 0.2 M ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K