3TYW

Crystal Structure of CYP105N1 from Streptomyces coelicolor A3(2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-01-27
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97
Spacegroup name	P 61
Unit cell lengths	134.537, 134.537, 230.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.2821
Rwork	0.282
R-free	0.30050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.091	0.139
Number of reflections	51760
<I/σ(I)>	26.8	6.1
Completeness [%]	99.8	99.8
Redundancy		9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	PEG 3350, HEPES 7.0, Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 295K