3TYJ
Bacillus collagen-like protein of anthracis P159S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 68.152, 68.152, 163.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.080 - 2.150 |
| R-factor | 0.20865 |
| Rwork | 0.207 |
| R-free | 0.23395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (for MRr) |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 12588 | |
| <I/σ(I)> | 11.7 | 3.2 |
| Completeness [%] | 96.9 | 97.9 |
| Redundancy | 11.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.02 | 295 | 0.7M ammonium phosphate monobasic, 100mM sodium citrate, pH 5.02, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
