3TY4
Crystal structure of homoisocitrate dehydrogenase from Schizosaccharomyces pombe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.851, 94.452, 76.076 |
Unit cell angles | 90.00, 106.63, 90.00 |
Refinement procedure
Resolution | 24.860 - 1.550 |
R-factor | 0.1893 |
Rwork | 0.188 |
R-free | 0.21550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ty3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.409 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmerge | 0.055 | 0.035 | 0.459 |
Number of reflections | 103345 | ||
<I/σ(I)> | 15.3 | ||
Completeness [%] | 99.3 | 99.6 | 94.2 |
Redundancy | 6.9 | 7.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3550, Li acetate, vapor diffusion, hanging drop, temperature 293K |