3TXS
Crystal Structure of phage 44RR small terminase gp16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | H 3 |
Unit cell lengths | 140.605, 140.605, 53.639 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.360 - 1.810 |
R-factor | 0.18866 |
Rwork | 0.187 |
R-free | 0.21990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3txq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.360 | 1.870 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.055 | 0.392 |
Number of reflections | 35747 | |
<I/σ(I)> | 25 | 5 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 5.8 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 12.5% PEG3350, 0.1M NaCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |