3TXN

Crystal structure of Rpn6 from Drosophila melanogaster, native data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-03
Detector	PILATUS 6M
Wavelength(s)	1.00686
Spacegroup name	P 61
Unit cell lengths	161.251, 161.251, 42.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.496
R-factor	0.2182
Rwork	0.216
R-free	0.26500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.294
Data reduction software	XDS
Data scaling software	SCALA (3.2.25)
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.028	52.782	2.630
High resolution limit [Å]	2.496	7.890	2.496
Rmerge	0.046	0.029	0.303
Total number of observations		2278	10599
Number of reflections	22030
<I/σ(I)>	14.4	20.4	2.5
Completeness [%]	99.3	98	99
Redundancy	3.3	3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	100 mM Tris HCl pH 7.5 200 mM Li2SO4, 12% PEG-3350, vapor diffusion, temperature 291K