3TXM
Crystal structure of Rpn6 from Drosophila melanogaster, Gd(3+) complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0332, 1.71024, 1.71072 |
| Spacegroup name | P 61 |
| Unit cell lengths | 161.251, 161.251, 42.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.25130 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 140.218 | 38.890 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.048 | 0.018 | 0.489 |
| Total number of observations | 1260 | 6532 | |
| Number of reflections | 12435 | ||
| <I/σ(I)> | 14.5 | 35.4 | 1.6 |
| Completeness [%] | 97.9 | 90.6 | 96.6 |
| Redundancy | 3.6 | 3.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 100 mM Tris HCl pH 7.5 200 mM Li2SO4, 12% PEG-3350, vapor diffusion, temperature 291K |
