3TXM

Crystal structure of Rpn6 from Drosophila melanogaster, Gd(3+) complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0332, 1.71024, 1.71072
Spacegroup name	P 61
Unit cell lengths	161.251, 161.251, 42.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.000
R-factor	0.2076
Rwork	0.205
R-free	0.25130
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	SCALA (3.2.25)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.218	38.890	3.160
High resolution limit [Å]	3.000	9.490	3.000
Rmerge	0.048	0.018	0.489
Total number of observations		1260	6532
Number of reflections	12435
<I/σ(I)>	14.5	35.4	1.6
Completeness [%]	97.9	90.6	96.6
Redundancy	3.6	3.1	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	100 mM Tris HCl pH 7.5 200 mM Li2SO4, 12% PEG-3350, vapor diffusion, temperature 291K