3TX6
The Structure of a putative ABC-transporter periplasmic component from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921, 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.564, 68.668, 90.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.14 |
| Rwork | 0.137 |
| R-free | 0.19810 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.696 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.075 | 0.062 | 0.459 |
| Number of reflections | 49224 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.8 | 97.3 | 99.9 |
| Redundancy | 8.6 | 8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1M Bis-Tris Propane:NaOH pH 7.0, 2.5M Amonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
