3TX3
CysZ, a putative sulfate permease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.7432 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.853, 81.986, 100.375 |
| Unit cell angles | 90.00, 125.12, 90.00 |
Refinement procedure
| Resolution | 29.172 - 2.300 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.23760 |
| Structure solution method | Native SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 38075 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, pH 6.0, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, pH 6.0, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
