3TWH
Selenium Derivatized RNA/DNA Hybrid in complex with RNase H Catalytic Domain D132N Mutant
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9797, 0.9800, 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.660, 37.819, 61.937 |
Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.790 |
R-factor | 0.17714 |
Rwork | 0.175 |
R-free | 0.20782 |
Structure solution method | MAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.372 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.530 | 1.860 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.060 | 0.278 |
Number of reflections | 17227 | |
<I/σ(I)> | 15.3 | |
Completeness [%] | 98.6 | 98.6 |
Redundancy | 7.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES, 12% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |