3TW1
Structure of Rtt106-AHN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-05 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.517, 91.845, 34.824 |
| Unit cell angles | 90.00, 105.12, 90.00 |
Refinement procedure
| Resolution | 32.173 - 1.772 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fss |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.549 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.051 | 0.560 |
| Number of reflections | 22670 | |
| <I/σ(I)> | 26.6 | 4.2 |
| Completeness [%] | 93.0 | 83.7 |
| Redundancy | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 4% v/v tacsimate, 12% PEG3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
