3TVZ
Structure of Bacillus subtilis HmoB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97865 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.799, 64.799, 392.591 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.740 - 2.000 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 34657 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.5 | 293 | 28% PEG 4000, 0.2M Magnesium chloride, 0.1M Tris-Hcl pH8.5, hanging drop, temperature 293K |
| 1 | hanging drop | 8.5 | 293 | 28% PEG 4000, 0.2M Magnesium chloride, 0.1M Tris-Hcl pH8.5, hanging drop, temperature 293K |
