3TVV
Structure of the tandem PH domains of Rtt106 (residues 68-315)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.660, 60.640, 77.170 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 31.307 - 2.589 |
| R-factor | 0.2408 |
| Rwork | 0.238 |
| R-free | 0.29420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fss |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.648 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_886) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.660 |
| High resolution limit [Å] | 2.563 | 2.563 |
| Rmerge | 0.098 | 0.450 |
| Number of reflections | 19166 | |
| <I/σ(I)> | 18.1 | 2.1 |
| Completeness [%] | 93.9 | 66.3 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 8% v/v tacsimate, 20% PEG3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
