3TV0
Structure of dynactin p27 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.960, 57.140, 80.190 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 43.451 - 2.149 |
| R-factor | 0.2163 |
| Rwork | 0.214 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.500 | 2.190 |
| High resolution limit [Å] | 2.149 | 2.149 |
| Number of reflections | 16255 | |
| Completeness [%] | 81.1 | 38.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.6 | 295 | 19% PEG 2000, 0.15 M Potassium phosphate, pH 3.6, VAPOR DIFFUSION, temperature 295K |
