3TUS
Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Meta-hydroxy benzoic acid at 2.5 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.431, 50.492, 65.404 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 39.260 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ib0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.260 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 12573 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 92.2 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
