3TUL
Crystal structure of N-terminal region of Type III Secretion Major Translocator SipB (residues 82-226)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97243,0.97934 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 159.154, 51.144, 84.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.943 - 2.793 |
R-factor | 0.299 |
Rwork | 0.298 |
R-free | 0.31640 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.943 | 2.968 |
High resolution limit [Å] | 2.793 | 2.793 |
Rmerge | 0.100 | 0.671 |
Number of reflections | 17478 | |
<I/σ(I)> | 18.8 | 2.55 |
Completeness [%] | 98.0 | 80 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 0.15 M potassium bromide, 27% PEG2000 MME, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |