3TUF
Structure of the SpoIIQ-SpoIIIAH pore forming complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 78.797, 111.088, 136.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.720 - 2.260 |
| R-factor | 0.16858 |
| Rwork | 0.165 |
| R-free | 0.22581 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.083 | 0.410 |
| Number of reflections | 23724 | |
| <I/σ(I)> | 25.7 | 2.1 |
| Completeness [%] | 83.0 | 32.1 |
| Redundancy | 6.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1 M sodium acetate,50% PEG 400, 0.5 mM spermidine, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
