3TTS
Crystal structure of beta-galactosidase from Bacillus circulans sp. alkalophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97240 |
| Spacegroup name | H 3 |
| Unit cell lengths | 226.000, 226.000, 246.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.337 - 2.401 |
| R-factor | 0.1549 |
| Rwork | 0.152 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kwg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 182304 | |
| Completeness [%] | 99.3 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 6-9% PEG 3350, 0.1M magnesium sulfate, 0.1M bicine, pH 8,5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
