3TTR
Crystal structure of C-lobe of bovine lactoferrin complexed with Lidocaine at 2.27 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 300 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-05 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.671, 50.421, 65.887 |
Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
Resolution | 39.340 - 2.270 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o97 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.800 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 2.350 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 17966 | |
<I/σ(I)> | 9.3 | 2 |
Completeness [%] | 94.8 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |