3TTK
Crystal structure of apo-SpuD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-15 |
Detector | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.769, 108.983, 228.508 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.970 |
R-factor | 0.21876 |
Rwork | 0.215 |
R-free | 0.28794 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ttm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.207 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 23272 | |
Completeness [%] | 84.9 | 80.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 25% PEG 3350, 0.1M Mes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |