3TTG
Crystal structure of putative aminomethyltransferase from Leptospirillum rubarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-19 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 115.616, 115.616, 75.428 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.560 - 2.000 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.099 | 0.673 |
| Number of reflections | 34441 | |
| <I/σ(I)> | 23.5 | 3 |
| Completeness [%] | 98.3 | 94.4 |
| Redundancy | 11.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 296 | 0.5 M K3PO4/0.5 M Na3PO4, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
