3TT6

Structure of ClpP from Bacillus subtilis in compressed state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	109.090, 172.864, 83.427
Unit cell angles	90.00, 118.93, 90.00

Refinement procedure

Resolution	36.088 - 2.592
R-factor	0.2238
Rwork	0.221
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ce3
RMSD bond length	0.013
RMSD bond angle	1.354
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.592	5.600	2.600
Rmerge	0.072	0.049	0.503
Number of reflections	41517
<I/σ(I)>	13.6
Completeness [%]	99.4	99.3	94.6
Redundancy	3.6	3.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	5.6	295	0.1M sodium citrate, 0.1M lithium sulfate, 10-12%(w/v) PEG 4000, pH 5.6, hanging drop, temperature 295K