3TSQ
Crystal structure of E. coli HypF with ATP and Carbamoyl phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-04 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.28248 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.530, 78.287, 200.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.410 - 2.400 |
R-factor | 0.1577 |
Rwork | 0.154 |
R-free | 0.23282 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.850 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.410 |
High resolution limit [Å] | 2.400 |
Number of reflections | 29549 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M HEPES, pH 8.5, 20 mM magnesium chloride, 2.5% w/v isopropanol, 10% w/v ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |