3TSB
Crystal Structure of Inosine-5'-monophosphate Dehydrogenase from Bacillus anthracis str. Ames
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 4 |
| Unit cell lengths | 123.026, 123.026, 140.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.997 - 2.595 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1ZFJ monomer |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.595 | 2.600 |
| Number of reflections | 31165 | |
| <I/σ(I)> | 10.6 | 2.6 |
| Completeness [%] | 97.0 | 98.4 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 1.0 M NaH2PO4/K2HPO4 pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
