3TS2
Mouse Lin28A in complex with let-7g microRNA pre-element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.030, 109.210, 182.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.820 - 2.010 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3trz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.410 | 2.014 |
| High resolution limit [Å] | 2.007 | 2.007 |
| Rmerge | 0.046 | 0.433 |
| Number of reflections | 30861 | |
| <I/σ(I)> | 16.9 | 2.1 |
| Completeness [%] | 98.3 | 92.5 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.1M Tris pH 8.0, 32% w/v PEG 4000, and 0.2M sodium acetate , VAPOR DIFFUSION, temperature 298K |
