3TS0
Mouse Lin28A in complex with let-7f-1 microRNA pre-element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 139.880, 139.880, 85.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.980 - 2.763 |
R-factor | 0.1941 |
Rwork | 0.193 |
R-free | 0.21450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3trz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.980 | 2.772 |
High resolution limit [Å] | 2.763 | 2.763 |
Rmerge | 0.060 | 0.629 |
Number of reflections | 22328 | |
<I/σ(I)> | 28.2 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 0.6M NaH2PO4, 1.4M K2HPO4, pH 7, vapor diffusion, temperature 298K |