3TRE
Structure of a translation elongation factor P (efp) from Coxiella burnetii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 82.619, 82.619, 79.096 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.310 - 2.899 |
| R-factor | 0.2371 |
| Rwork | 0.235 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yby |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.899 | 7.860 | 2.900 |
| Rmerge | 0.067 | 0.053 | 0.531 |
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 98.8 | 97.2 | 99.9 |
| Redundancy | 2.8 | 4.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.05M potassium thiocyanate 16% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
