3TQ7

EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-07-29
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	41.150, 103.560, 48.130
Unit cell angles	90.00, 113.64, 90.00

Refinement procedure

Resolution	44.090 - 2.300
R-factor	0.229
Rwork	0.226
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wu9
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.095	20.000	2.400
High resolution limit [Å]	2.300	4.000	2.300
Number of reflections	15694
<I/σ(I)>	27.64	57.88	5.42
Completeness [%]	95.2	99.2	99.1
Redundancy	13.4	13	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	15% PEG 4000, 0.1M Tris-HCl, 0.2M Magnesium chloride hexahydrate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K