3TPQ
Crystal structure of wild-type MAL RPEL domain in complex with five G-actins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-26 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 180.737, 180.737, 382.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.700 - 3.450 |
| R-factor | 0.2268 |
| Rwork | 0.224 |
| R-free | 0.27330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v52 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.729 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.720 | 3.640 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Number of reflections | 41449 | |
| Completeness [%] | 83.9 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.3 | 293 | 50mM citrate, 0.1M ammonium sulfate, 0.1mM CaCl2, 0.1mM ATP, 10% PEG8000, pH 5.3, VAPOR DIFFUSION, temperature 293K |
