3TPB
Structure of HipA(S150A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.430, 84.500, 50.000 |
| Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.880 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.500 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 29360 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 14% PEG 8000, 20% glycerol and 0.04 M potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
