3TP8
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.226, 60.621, 38.186 |
| Unit cell angles | 90.00, 92.96, 90.00 |
Refinement procedure
| Resolution | 38.140 - 1.600 |
| R-factor | 0.1851 |
| Rwork | 0.184 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.046 | 0.053 | 0.155 |
| Number of reflections | 22377 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 98.1 | 99.7 | 84 |
| Redundancy | 7.8 | 9.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
