3TP2

Crystal Structure of the Splicing Factor Cwc2 from yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	58.700, 79.700, 113.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.260 - 2.400
R-factor	0.2231
Rwork	0.221
R-free	0.25550
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.927
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.264		2.300
High resolution limit [Å]	2.200	10.000	2.200
Rmerge	0.082	0.042	0.427
Number of reflections	27711	343	3225
<I/σ(I)>	21.8	42.53	5.51
Completeness [%]	99.3	98.8	94.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG5000 MME, ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K