3TP2
Crystal Structure of the Splicing Factor Cwc2 from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.700, 79.700, 113.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.260 - 2.400 |
| R-factor | 0.2231 |
| Rwork | 0.221 |
| R-free | 0.25550 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.927 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.264 | 2.300 | |
| High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
| Rmerge | 0.082 | 0.042 | 0.427 |
| Number of reflections | 27711 | 343 | 3225 |
| <I/σ(I)> | 21.8 | 42.53 | 5.51 |
| Completeness [%] | 99.3 | 98.8 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG5000 MME, ammonium sulfate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
