3TOW
Crystal Structure of the MABP Domain of MVB12B of Human ESCRT-I Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.371, 52.607, 71.388 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.537 - 1.340 |
R-factor | 0.1663 |
Rwork | 0.165 |
R-free | 0.19310 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.465 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.079 | 0.520 |
Number of reflections | 27812 | |
<I/σ(I)> | 9.9 | 2.017 |
Completeness [%] | 99.5 | 93.3 |
Redundancy | 7.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 33% PEG 400, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 33% PEG 400, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |