3TOR
Crystal structure of Escherichia coli NrfA with Europium bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.884, 90.489, 292.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 146.100 - 2.000 |
| R-factor | 0.16646 |
| Rwork | 0.164 |
| R-free | 0.22194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rdz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.854 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.100 | 2.053 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 139682 | |
| <I/σ(I)> | 11.6 | 8.73 |
| Completeness [%] | 98.5 | 93.28 |
| Redundancy | 3.01 | 2.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Crystals were obtained in 100 mM HEPES pH 7.5, 20 % PEG 10 K. Soaked in mother liqour containing 10 mM Eu(II)Cl, 20 % Ethylene glycol for 2 minutes before immersion in liquid nitrogen., VAPOR DIFFUSION, HANGING DROP, temperature 277K |
