3TO5
High resolution structure of CheY3 from Vibrio cholerae
Experimental procedure
Experimental method | LAUE |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 68.057, 68.057, 75.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.260 - 1.650 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2che |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 14876 | |
Completeness [%] | 94.3 | 91.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 6000, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |