3TN6
Crystal structure of GkaP mutant R230H from Geobacillus kaustophilus HTA426
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.147, 88.638, 89.718 |
| Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| Rwork | 0.210 |
| R-free | 0.22529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3orw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.074 | 0.057 | 0.449 |
| Number of reflections | 103769 | ||
| <I/σ(I)> | 15.8 | ||
| Completeness [%] | 99.7 | 97.7 | 100 |
| Redundancy | 5.5 | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 287 | 13% PEG 8000, 8% ethylene glycol, 2% glycerol, 0.05M sodium hepes pH7.3, vapor diffusion, hanging drop, temperature 287K |
