3TN3
Crystal structure of GkaP from Geobacillus kaustophilus HTA426
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.307, 88.146, 89.994 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 44.070 - 1.600 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3orw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 99136 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 287 | 13% PEG 8000, 8% ethylene glycol, 2% glycerol, 0.05M sodium hepes pH7.3, vapor diffusion, hanging drop, temperature 287K |
