3TMO
The catalytic domain of human deubiquitinase DUBA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 64 |
Unit cell lengths | 66.658, 66.658, 82.218 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.860 - 2.200 |
R-factor | 0.24459 |
Rwork | 0.243 |
R-free | 0.27615 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.011 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 21875 | |
<I/σ(I)> | 19.9 | 3 |
Completeness [%] | 98.2 | 83.8 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8 M succinic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |