3TMC

Bd1817, a HDG"Y"P protein from Bdellovibrio bacteriovorus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	47.100, 49.401, 78.372
Unit cell angles	73.17, 75.90, 67.34

Refinement procedure

Resolution	13.669 - 1.550
R-factor	0.2108
Rwork	0.209
R-free	0.23550
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.029
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.700	1.630
High resolution limit [Å]	1.550	1.550
Rmerge	0.080
Number of reflections	80548
<I/σ(I)>	13.4
Completeness [%]	90.0	62.9
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1M Bis Tris pH 5.5, 0.4M Ammonium Acetate, 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K