3TMA

Crystal structure of TrmN from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 32 2 1
Unit cell lengths	65.860, 65.860, 144.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.770 - 2.050
R-factor	0.15707
Rwork	0.154
R-free	0.21176
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tlj
RMSD bond length	0.016
RMSD bond angle	1.516
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.101	0.525
Number of reflections	23534
<I/σ(I)>	22.4	7.37
Completeness [%]	100.0	100
Redundancy	21.3	22.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	100 mM citrate/phosphate, 15% PEG 6000, 200 mM NaCl, 100 mM sodium citrate, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K