3TLZ
Microcin C7 self immunity protein MccF mutant W186F in complex with Adenosine Monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Collection date | 2010-06-05 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.553, 90.189, 73.322 |
Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.500 |
R-factor | 0.17405 |
Rwork | 0.173 |
R-free | 0.19272 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tla |
RMSD bond length | 0.006 |
RMSD bond angle | 1.015 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.800 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.067 | 0.261 |
Number of reflections | 115009 | |
<I/σ(I)> | 38.094 | 5.593 |
Completeness [%] | 97.8 | 95 |
Redundancy | 5.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 282 | 0.1M HEPES pH 7.5, 10% PEG 8000, 8% Ethylene Glycol, vapor diffusion, temperature 282K, VAPOR DIFFUSION |