3TLM
Crystal Structure of Endoplasmic Reticulum Ca2+-ATPase (SERCA) From Bovine Muscle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1271 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.580, 75.260, 151.800 |
| Unit cell angles | 90.00, 108.30, 90.00 |
Refinement procedure
| Resolution | 41.990 - 2.950 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t5s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.610 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.106 | 0.692 |
| Number of reflections | 35546 | |
| <I/σ(I)> | 14.1 | 3.5 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 220mM sodium acetate, 4% t-butanol, 5mM mercaptoethanol, 15% glycerol, 6-7% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
