3TLG
Microcin C7 self immunity protein MccF in the inactive mutant APO state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.507, 85.808, 73.198 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 26.133 - 1.499 |
R-factor | 0.1628 |
Rwork | 0.161 |
R-free | 0.19010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.068 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
High resolution limit [Å] | 1.499 | 3.230 | 1.499 |
Rmerge | 0.069 | 0.059 | 0.121 |
Number of reflections | 205542 | ||
<I/σ(I)> | 20.5 | ||
Completeness [%] | 99.7 | 98.1 | 99.8 |
Redundancy | 4.6 | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 282 | vapor diffusion, temperature 282K |