3TL4
Crystal Structure of the tRNA Binding Domain of Glutaminyl-tRNA Synthetase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-14 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97904, 0.91162, 0.97937 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.800, 34.620, 74.269 |
Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
Resolution | 26.299 - 2.300 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21100 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.034 |
Data scaling software | SCALA (3.3.16) |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 73.615 | 26.299 | 2.420 |
High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
Rmerge | 0.033 | 0.490 | |
Total number of observations | 1866 | 9002 | |
Number of reflections | 9393 | ||
<I/σ(I)> | 13 | 19.2 | 1.6 |
Completeness [%] | 99.9 | 98.3 | 100 |
Redundancy | 6.5 | 5.8 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch under oil | 8 | 295 | 100mM KCl, 100mM Tris, 20% (w/v) PEG 4000, pH 8.0, microbatch under oil, temperature 295K |