3TJY
Structure of the Pto-binding domain of HopPmaL generated by limited chymotrypsin digestion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.427, 57.427, 54.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.682 - 1.700 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.21740 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.682 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.501 |
| Number of reflections | 11555 | |
| <I/σ(I)> | 42.133 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M Bis-Tris pH 6.5 plus 1.5M Ammonium Sulphate. 0.03 mg/ml chymotrypsin was added to the protein prior to adding crystallization liquor. Crystals were cryoprotected with Paratone-N oil , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
