3TJL
Crystal Structure of a Novel OYE from the Xylose-fermenting Fungus P. stipitis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 127.164, 127.164, 122.457 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.000 - 1.500 |
| R-factor | 0.1454 |
| Rwork | 0.143 |
| R-free | 0.16820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oya |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: dev_845)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.000 |
| High resolution limit [Å] | 1.200 |
| Rmerge | 0.056 |
| Number of reflections | 92358 |
| Completeness [%] | 1.0 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2.4M Sodium malonate 1-3% 2-propanol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
