3TJK
Crystal Structure of human peroxiredoxin IV C245A mutant in reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.240, 138.400, 96.000 |
| Unit cell angles | 90.00, 103.80, 90.00 |
Refinement procedure
| Resolution | 83.710 - 2.090 |
| R-factor | 0.16247 |
| Rwork | 0.160 |
| R-free | 0.20125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.598 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.710 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.053 | 0.683 |
| Number of reflections | 80529 | |
| <I/σ(I)> | 14.3 | 2.4 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.0 M succinic acid, 1% w/v PEG2000 MME, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
