3TJ4
Crystal structure of an enolase from agrobacterium tumefaciens (efi target efi-502087) no mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 118.739, 118.739, 113.423 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.500 |
| R-factor | 0.1489 |
| Rwork | 0.148 |
| R-free | 0.16540 |
| Structure solution method | MIR |
| Starting model (for MR) | 3cb3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.074 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.069 | 0.320 |
| Number of reflections | 127613 | |
| <I/σ(I)> | 22.2 | 4.6 |
| Completeness [%] | 98.8 | 98.8 |
| Redundancy | 8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | Protein (10 mM Hepes, pH 7.8, 150 mM NaCl, 10% glycerol, 5 mM DTT, 5 mM MgCl2; Reservoir (0.4 M AmPO4 monobasic); Cryoprotection (Reservoir + 20% glycerol), sitting drop vapor diffuction, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
