3TIR
Pseudo-atomic model of the Rous Sarcoma Virus capsid hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-10 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 6 |
| Unit cell lengths | 92.559, 92.559, 49.985 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.960 - 4.100 |
| Rwork | 0.394 |
| R-free | 0.39100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1EM9 AND 3G1I. |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 4.220 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.122 | 0.316 |
| Number of reflections | 1996 | |
| <I/σ(I)> | 5.6 | 1.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.6 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.3 | 291.15 | 2-7 %(w/v) PEG 8000 0.2M CAPS/KOH, pH 10.3, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
